Composite

Part:BBa_K4658005:Design

Designed by: Tanvi Garg   Group: iGEM23_Stockholm   (2023-10-07)


Anchor Peptide with EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 224
    Illegal NotI site found at 239
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The major design considerations were the positions of start and stop codon with respect to the His-tag on the backbone, and the restriction enzymes required.



Source

This sequence has been designed in the lab and does not come from a genomic sequence. Although, it consists of different parts that come from various genomic sources.


References

Rübsam et al. (2017)